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KMID : 0361919950250030333
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Korean Journal of Orthodontics
1995 Volume.25 No. 3 p.333 ~ p.339
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THE EFFECT OF 1.25-(OH)2D3 ON THE PROLIFERATION AND ALKALINE PHOSPHATASE ACTIVITY OF HUMAN PERIODONTAL LIGAMENT CELLS
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Abstract
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The hormonally active vitamin D metabolite, 1,25-dihydrouy vitamin D3 [1.25{OH)2D3J is one of the several humoral factors that may regulate osteoblast differentiation. The purpose of this study was to evaluate the effects of L25{OH) % on the PDL cells. Human PDL cells were prepared from the first premolar tooth extracted for the orthodontic treatment and they were incubated in the environment of 37°C, 5% C02 and 95% humidity.
[3M -tbymidine incorporation as a measure of proliferation potential and alkaline phosphatase activity were evaluated at 10n! , 100nM 1,25-(OH)2Da
The observed results were as follows.
1. 1,25.(OH)2D3 was significantly enhanced [3H]-thymidine incorporation at 1OOnI But did not affect by lOnAC
2. 1;2540H)21)3 was significantly increased alkaline phosphatase activity at 1 day and 6 days in a dose-dependent manner.
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